An electrochemical DNA sensor for determination of 6-thioguanine using adsorptive stripping voltammetry at HMDE: An anticancer drug DNA interaction study
Elham Mirmomtaz, Ali Zirakbash, Ali Asghar Ensafi
Российский электрохимический журнал
https://doi.org/10.1134/S1023193516040078
In the present study, the electrochemical behavior of 6-Thioguanine (6-TG) and its interaction with double-strand DNA (ds-DNA) were investigated at the surface of hanging mercury drop electrode (HMDE) in neutral media. The interaction of 6-TG with ds-DNA in neutral buffer was clearly demonstrated by the elimination of 6-TG signal and the advent of a new reduction peak. To investigate the interaction, various parameters including accumulation time and potential as well as ds-DNA concentration were optimized using a combination of adsorptive stripping voltammetry (AdSV) and square wave voltammetry (SWV) techniques. As a consequence a low detection limit of 1.1 μM was obtained in a dynamic range of 16.0 to 360.0 μM. To better understand the interaction mechanism between 6-TG anti-cancer drug and ds-DNA, cyclic voltammetry and UV-Vis spectroscopy measurements were carried out and the intercalation of 6-TG into ds-DNA was proposed as the plausible mechanism. The application of this screening assay in real sample analysis was investigated by using the procedure for determination of 6-TG in 6-Thioguanine tablets and also in spiked 6-TG blood serum. Overall, the results were indicative of a DNA sensor which could be applied effectively in the analysis of 6-TG in vitro.
- Department of Science, Islamic Azad University, Shahrekord Branch, Shahrelcord, 88137, IranElham Mirmomtaz
- Department of Chemistry, Islamic Azad University, Shahreza Branch, Shahreza, 86145, IranAli Zirakbash
- Department of Chemistry, Isfahan University of Technology, Isfahan, 84156, IranAli Asghar Ensafi
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