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Статья
2020

Kinetics of Mediated Bioelectrocatalytic Oxidation of Glucose by Protein Extracts of Escherichia coli

M. V. DmitrievaM. V. Dmitrieva, I. N. ShishovI. N. Shishov, S. V. ShmaliiS. V. Shmalii, V. D. MyazinV. D. Myazin, A. Yu. BazhenovA. Yu. Bazhenov, E. V. GerasimovaE. V. Gerasimova, E. V. ZolotukhinaE. V. Zolotukhina
Российский электрохимический журнал
https://doi.org/10.1134/S1023193520110038
Abstract / Full Text
Dmitrieva, M.V., Shishov, I.N., Shmalii, S.V. et al. Kinetics of Mediated Bioelectrocatalytic Oxidation of Glucose by Protein Extracts of Escherichia coli . Russ J Electrochem 56, 938–945 (2020). https://doi.org/10.1134/S1023193520110038

The kinetics of bioelectrocatalytic oxidation of glucose by protein extracts—supersonic-destruction products of Escherichia coli BB cells—is studied in the presence of [Fe(CN)6]3– as the mediator system. The effect of the concentration of mediator, glucose, and protein extract is studied by electrochemical methods. The results are used in determination of effective parameters: the rate constant of glucose biooxidation, the constant of substrate-induced inhibition, and the activation energy. It is shown that the activation energy of this reaction falls into the interval of activation energies of dehydrogenase reactions. The voltammetric characteristics of a model asymmetrical biofuel cell which employs the protein extract as the anodic catalyst are determined. The maximum specific power of such model biofuel cell is found to be 400 µW/cm2 (4 W/m2).

Author information
  • Institute of Problems of Chemical Physics, Russian Academy of Sciences, 142432, Chernogolovka, Moscow oblast, RussiaM. V. Dmitrieva, E. V. Gerasimova & E. V. Zolotukhina
  • Moscow State University, Leninskie Gory, 119992, Moscow, RussiaI. N. Shishov, S. V. Shmalii, V. D. Myazin & A. Yu. Bazhenov
  • Moscow Institute of Physics and Technology, 141701, Dolgoprudnyi, Moscow oblast, RussiaE. V. Zolotukhina
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