Fast and Sensitive Detection of Diisononyl Phthalate in Liquor Sample by Molecularly Imprinted Polymer Based Electrochemical Sensor

X. Zhao X. Zhao , X. Ju X. Ju , S. Qiu S. Qiu , W. Hu W. Hu , L. Yang L. Yang , J. Zhang J. Zhang
Российский электрохимический журнал
Abstract / Full Text

A novel electrochemical sensor for diisononyl phthalate (DINP) analysis was investigated. The sensor was modified on a glassy carbon electrode (GCE) with DINP molecularly imprinted polymer particles (MIPs). The electrode was assembled by the mixture of MIPs and agarose in proportion. The MIPs were formed by bulk polymerization via non-covalent multiple interactions, which were further characterized by scanning electron microscopy (SEM). The linear response range of the MIP sensor was between 50 and 1000 nM, and the limit of detection (LOD) was 27 nM. The proposed system has the superiority of high-speed real-time detection capability, no sample pretreatment, simple operation process, little detection cost, short detection time, high sensitivity, low interference and good stability. Therefore, it shows the potential for application in food safety supervision of DINP.

Author information
  • Tianjin Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, TEDA, Tianjin, P. R. China

    X. Ju, S. Qiu & J. Zhang

  • College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, TEDA, Tianjin, P. R. China

    X. Zhao, W. Hu & L. Yang

  1. Julie, B., Sofie Ch., and Marta, A., Reproductive and behavioral effects of diisononyl phthalate (DINP) in perinatally exposed rats, Reprod. Toxicol., 2011, vol. 31, p.200.
  2. ECHA, DINP Review Report, Evaluation of new scientific evidence concerning the restrictions contained in Annex XVII to regulation (EC), no. 1907/2006(REACH), Review of new available information for diisononyl phthalate (DINP), 2010.
  3. Ruth, S., Iryna, L., and David, S.U., Concentrations of phthalate esters and identification of other additives in PVC children’s toys, Environ. Sci. Pollut. Res., 2000, vol. 7, no. 1, p.27.
  4. McKee, R.H., El-hawari, M., and Stoltz, M., Absorption, disposition and metabolism of di-isononyl phthalate (DINP) in F-344 rats, J. Appl. Toxicol., 2002, vol. 22, p.293.
  5. Kavlock, R., Boekelheide, K., and Chapin, R., NTP center for the evaluation of risks to human reproduction: phthalates expert panel report on the reproductive and develop-mental toxicity of di-isononly phthalate, Reprod. Toxicol., 2002, vol. 16, p.679.
  6. Kaufmann, W., Deckardt, K., and McKee, R.H., Tumor induction in mouse liver: di-isononyl phthalate acts via peroxisome proliferation, Regul. Toxicol. Pharmacol., 2002, vol. 36, p.175.
  7. Lington, A.W., Bird, M.G., and Plutnick, R.T., Chronic toxicity and carcinogenic evaluation of diisono-nyl phthalate in rats, Fundam. Appl. Toxicol., 1997, vol. 36, p.79.
  8. Gray, L.E., Jr., Joseph, O., and Johnathan, F., Perinatal exposure to the phthalates DEHP, BBP, and DINP, but not DEP, DMP, or DOTP, alters sexual differentitation of the male rat, Toxicol. Sci., 2000, vol. 58, p.350.
  9. Skakkebaek, N.E., Rajpert-De, M.E., and Main, K.M., Testicular dysgenesis syndrome: An increasingly common developmental disorder with environmental aspects, Hum. Reprod., 2001, vol. 16, no. 5, p.972.
  10. Chronic Health Advisory Panel, Report to the US Consumer Product Safety Commission by the Chronic Health Advisory Panel on Diisononyl Phthalate (DINP), 2001.
  11. Consumer Product Safety Improvement Act of 2008, Publ. L., 2008, vol. 3016, no. 122, p.110.
  12. https://doi.org/www.china.com.cn/news/tw/2011-05/30/content_22673877.htm.
  13. https://doi.org/www.chinanews.com/hb/2011/05-30/3076419.shtml.
  14. https://doi.org/finance.china.com.cn/consume/puguang/20121119/1140854.shtml.
  15. https://doi.org/news.cnhubei.com/xw/jj/201211/t2329274.shtml.
  16. https://doi.org/news.qq.com/a/20130302/000949.htm.
  17. https://doi.org/www.cnr.cn/gundong/201303/t20130303_512068541.shtml.
  18. Marie, L., Bertrand, D., and Yoann, G., Comparison of high-performance liquid chromatography and supercritical fluid chromatography using evaporative light scattering detection for the determination of plasticizers in medical devices, J. Chromatogr. A, 2015, vol. 1417, p.104.
  19. Dunming, X., Xiaojun, D., and Enhua, F., Determination of 23 phthalic acid esters in food by liquid chromatography tandem mass spectrometry, J. Chromatogr. A, 2014, vol. 1324, p.49.
  20. Paz, O., Sushanta, K.S., and Siobhan, M., Improved method for rapid detection of phthalates in bottled water by gas chromatography–mass spectrometry, J. Chromatogr. B, 2015, vol. 997, p.229.
  21. Earls, A.O., Axford, I.P., and Braybrook, J.H., Gas chromatography–mass spectrometry determination of the migration of phthalate plasticisers from polyvinyl chloride toys and childcare articles, J. Chromatogr. A, 2003, vol. 983, p.237.
  22. Ioannis, I., Alexandros, L., and Eugenia, L., Determination of phthalates into vegetable oils by isotopic dilution gas chromatography mass spectrometry, Food Anal. Methods, 2014, vol. 7, p. 1451.
  23. Kayoko, K., Manori, J.S., and Larry, L.N., Determination of total phthalates in urine by isotope-dilution liquid chromatography–tandem mass spectrometry, J. Chromatogr. B, 2005, vol. 814, p.355.
  24. Juankun, Zh., Min, L., and Peidong, X., Directly fast detection of digoxin in the serum sample by the synthetic receptor sensor, Mater. Sci. Eng., B, 2013, vol. 178, p. 1191.
  25. Jian, J., Zhihui, Zh., and Xiaolian, Zh., Electrochemical sensor based on molecularly imprintedfilm at Au nanoparticles-carbon nanotubes modified electrode for determination of cholesterol, Biosens. Bioelectron., 2015, vol. 66, p.590.
  26. Hui, L., Wanzhen, X., and Ningwei, W., Synthesis of magnetic molecularly imprinted polymer particles for selective adsorption and separation of dibenzothiophene, Microchim. Acta, 2012, vol. 179, p.123.
  27. Xinlong, X., Edward, P.C.L., and Banu, Ör., Duomolecularly imprinted polymer-coated magnetic particles for class-selective removal of endocrine-disrupting compounds from aqueous environment, Environ. Sci. Pollut. Res., 2013, vol. 20, p. 3331.
  28. Ratna, K., Reinhard, S., and Sevil, Yü., Catalysis of a β-elimination applying membranes with incorporated molecularly imprinted polymer particles, Polym. Bull., 2005, vol. 55, p.287.
  29. Maiorova, N.A., Safonov, V.A., and Skundin, A.M., Electrochemical Frontiers in Global Environment and Energy (Survey of Materials of the 62nd Annual Meeting of the International Society of Electrochemistry), Russ. J. Electrochem., 2013, vol. 49, p.908.
  30. Fatiha, S. and Abdelkader, B., Electrochemical determination of Cd2+ at a titanium electrode modified with a lead film by square wave anodic stripping voltammetry, Russ. J. Electrochem., 2016, vol. 52, p. 27.