A sensitive and convenient electrochemical sensor was developed for determination of epinephrine by using the Fe3O4@SiO2/GR nanocomposite modified graphite screen printed electrode, and its electrochemical behaviour was investigated by cyclic voltammetry, chronoamperometry and differential pulse voltammograms. Differential pulse voltammetry results exhibited the linear dynamic range of 5.0–1000.0 μM, with detection limits (S/N = 3) of 1.0 μM. The prepared electrode was successfully applied for simultaneous determination of epinephrine and folic acid in real samples.