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Статья
2016

A novel electrochemical biosensor for detection of cholesterol


Lin XuLin Xu, Yiting HouYiting Hou, Mengdan ZhangMengdan Zhang, Xin YangXin Yang, Greath JenkinsGreath Jenkins, Wei HuangWei Huang, Cheng YaoCheng Yao, Qiong WuQiong Wu
Российский электрохимический журнал
https://doi.org/10.1134/S1023193516030101
Abstract / Full Text

We report on a highly sensitive electrochemical biosensor for determination of cholesterol. The biosensor was fabricated by co-immobilizing bi-enzymes, cholesterol oxidase (ChOx), and horseradish peroxidase (HRP). Voltammetric technique such as cyclic voltammetry and impedance experiment were used to study the characterization of modified electrode step by step. The developed sensor is cheap, disposable, portable and exhibits higher sensitivity. The biosensor expressed a wide linear range up to 300 mg dL–1 in a physiological condition (pH 7.0), with a correlation coefficient of 0.9969. A sensitivity of 13.28 μA mg–1 dL cm−2 which makes it very promising for the clinical determination of cholesterol.

Author information
  • State Key Laboratory of Materials-Oriented Chemical Engineering and College of Science, Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing, 211816, ChinaLin Xu & Cheng Yao
  • Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), National Jiangsu Synergistic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing, 211816, ChinaMengdan Zhang, Xin Yang, Wei Huang & Qiong Wu
  • Key Laboratory for Organic Electronics & Information Displays (KLOEID), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Yadong, Xincheng District, Nanjing, 210046, P. R. ChinaYiting Hou & Greath Jenkins
  • Nan Jing College of Chemical Technology, 625 GeGuan Road, Nanjing, 210048, ChinaLin Xu
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