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Article
2016

Tyrosine sensing on phthalic anhydride functionalized chitosan and carbon nanotube film coated glassy carbon electrode


Fereshteh ChekinFereshteh Chekin, Samira BagheriSamira Bagheri
Russian Journal of Electrochemistry
https://doi.org/10.1134/S1023193515120034
Abstract / Full Text

Phthaloylchitosan (PHCS) has been synthesized by a simple and low-cost method using chitosan and phthalic anhydride as organic precursors by microwave irradiation. Techniques of nuclear magnetic resonance (NMR), FT-IR spectroscopy and transmission electron microscope (TEM) were used to characterize the structure and properties of the Phthaloylchitosan. Moreover, glassy carbon electrode modified with Phthaloylchitosan and carbon nanotube (PHCS–CNT/GCE) was prepared by casting of the PHCS–CNT solution on GCE. The electrochemical behavior of PHCS–CNT/GCE was investigated and compared with the electrochemical behavior of Phthaloylchitosan modified GC (PHCS/GC), carbon nanotube modified GC (CNT/GC) and unmodified GC using cyclic voltammetry (CV). The Phthaloylchitosan film is electrochemically inactive; similar background charging currents are observed at bare GC. Electrochemical parameters, including apparent diffusion coefficient for the Fe(CN) 3-/4-6 redox probe at PHCS–CNT/GCE is comparable to values reported for GCE, CNT/GCE and PHCS/GCE. The PHCS–CNT/GCE sensor responded linearly to tyrosine (Tyr) in the concentration of 1.0 × 10–6 to 8.0 × 10–4 M with detection limit of 3.0 × 10–7 M at 3σ using amperometry. In addition, the PHCS–CNT/GCE displayed good reproducibility, high sensitivity and good selectivity towards the determination of Tyr, making it suitable for the determination of Tyr in clinical and medicine.

Author information
  • Department of Chemistry, Ayatollah Amoli Branch, Islamic Azad University, Amol, IranFereshteh Chekin
  • Centre for Research in Nanotechnology and Catalysis (NANOCEN), IPS Building, University of Malaya, 50603, Kuala Lumpur, MalaysiaSamira Bagheri
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