A trustworthy and simple electrode is fabricated using palladium nanoribbons for the determination of hemoglobin. Palladium nanoparticles were synthesised from M. acuminata fruit, which were self assembled to form palladium nanoribbons. Physical characterization of the modified electrode was carried out with the help of X-ray diffraction, scanning electron microscope and electrochemical impedance spectroscopy techniques. The electrochemical properties of the developed sensor towards the detection of hemoglobin were examined using cyclic voltammetry and chronoamperometry. By determining hemoglobin at ‒351 mV, the modified electrode confirms that it is highly sensitive. The response time and low detection limit is found to be 2 s and 0.039 µM, respectively, for hemoglobin. The proposed electrode also exhibits stability and reproducibility.